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Is it possible to use frozen primary CD34+ hematopoietic stem cells for Nucleofection?

2019-05-20 10:26:55 优者生物科技有限公司 阅读

Yes. 

For cryopreserved PBMCs or enriched CD34 populations, we recommend culturing the thawed cells 1-2 hours in culture medium before Nucleofection. Any further enrichment procedure after thawing is not recommended. Viability of frozen nucleofected cells might be lower than that of fresh ones.


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