优者生物科技有限公司

网站首页 > 技术支持 > 学习资料

How can I increase cell viability of Dendritic cells after Nucleofection?

2019-05-20 10:06:47 优者生物科技有限公司 阅读

DNA amount and quality are very critical for Nucleofection of Dendritic Cells (recommended 0.5-1µg; maximal 2 µg). LPS (lipopolysaccharides) have a strong negative effect on cell viability. Please make sure that all components you use for dendritic cell culture and transfection, e.g. PBS, FCS and especially DNA, are Endotoxin free. We additionally recommend purifying your DNA by precipitating it twice with 20% PEG/2.5 M NaCl. Viability is usually not an issue when working with mRNA or siRNA.


Powered by Youzre  ©2008-2019 www.youzre.com