Is there a special recommendation on Human T cell enrichment before Nucleofection?
For Nucleofection it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads after enrichment. Do not apply protocols using hypo-osmolar buffers. This may lead to high cell mortality after Nucleofection.