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Is there a special recommendation on Human T cell enrichment before Nucleofection?

2019-05-20 09:46:26 优者生物科技有限公司 阅读

For Nucleofection it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads after enrichment. Do not apply protocols using hypo-osmolar buffers. This may lead to high cell mortality after Nucleofection.


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