Why do you have different Optimized Protocols for the Nucleofection of unstimulated and stimulated Human T cells?
We found that the stimulation of T cells not only changes the cells' function but also significantly alters the requirements for successful Nucleofection.
That's why Lonza developed two Optimized Protocols for transfection giving different electrical parameters for stimulated and unstimulated T cells. In addition, the protocol is optimised for 5-6d stimulated T cells. If you use allostimulated or long term stimulated T cells like LAK cells, please contact our Scientific Support Team for assistance.